Date of Award
Master of Science (MS)
Nutrition, Dietetics, and Food Sciences
Jeffery R. Broadbent
Worldwide concern about food safety and associated health costs is increasing, and Salmonella contamination of foods is one of the most important causes of foodborne disease outbreaks. Although conventional detection methods for Salmonella offer high sensitivity and low cost, they require many different steps and are very time consuming. Ideally, methods to detect Salmonella in foods should be rapid, provide high specificity and sensitivity, be cost-effective, and low labor-intensive. Many rapid tests have been developed to address these goals, including DNA-based tests such as nucleic acid hybridization and PCR, immunoassay-based tests such as ELISA, ELF A and immunomagnetic method, and immuno-latex agglutination based tests. While these tests provide much faster results than conventional tests, additional work is needed to minimize the time required for Salmonella in food samples so that results are available within 1 day.
Attaphongse, Parichaya, "Methods for Detection of Salmonella spp." (2004). All Graduate Plan B and other Reports. 880.