Invasion by murine B16-F10 melanoma cells was studied using the human amniotic basement membrane (HABM) assay. B16-F10 cells were collected after a single passage through the amnion and grown to near confluency. The cycle of plating, passaging, collecting, and culturing B16-F10 cells was repeated five times. The invasion rate for B16-F10 cells remained relatively unchanged after six passages through the amnion. Injection of first-passage B16-F10 cells into C57BL6 mice resulted in 29 lung tumors per animal whereas sixth-passage cells resulted in 300+ lung tumors. While there exists no correlation of the number of cells penetrating the amnion with colonization number, lung colonization appears correlated with increased number of passages through the amnion.
Persky, B. and Chmielewski, G. W.
"A Comparative Study on in Vitro Invasion Rates by Melanoma Cells in the Human Amniotic Basement Membrane Model Versus in Vivo Tumor Nodule Formation in C57BL6 Mice,"
Scanning Microscopy: Vol. 1
, Article 43.
Available at: https://digitalcommons.usu.edu/microscopy/vol1/iss2/43