Scanning Microscopy
Abstract
Cryo-electron microscopy of single biological particles poses new challenges to digital image processing due to the low signal-to-noise ratio of the data. New tools have been devised to deal with important aspects of 3-D reconstruction following the random-conical data collection scheme: (a) a new shift-invariant function has been derived, which promises to facilitate alignment and classification of single particle projections; (b) a new method of orientation search is proposed, which makes it possible to relate random-conical data sets to one another prior to reconstruction; and (c) the foundation is laid for a 3-D variance estimation which utilizes the oversampling of 3-D angular space by projections in the random-conical reconstruction scheme.
Recommended Citation
Frank, Joachim; Penczek, Pawel; and Liu, Weiping
(1992)
"Alignment, Classification, and Three-Dimensional Reconstruction of Single Particles Embedded in Ice,"
Scanning Microscopy: Vol. 1992:
No.
6, Article 2.
Available at:
https://digitalcommons.usu.edu/microscopy/vol1992/iss6/2