Scanning Electron Microscopy of Intracellular Organelles in the Young Odontoblasts of Rats

Authors

Yasutomo Iwai-Liao, Osaka Dental University
Yoshikage Higashi, Osaka Dental University
Masakazu Ishikawa, Osaka Dental University
Hideaki Hori, Osaka Dental University

Abstract

Intracellular structures of the odontoblasts were studied by scanning electron microscopy (SEM) using a modified AODO (aldehyde prefixed-osmium-DMSO-osmium) method. Well-developed flattened and layered rER (rough endoplasmic reticulum), paved with its associated ribosomes on its outer surface, were clearly observed in the odontoblast. Branched tubular mitochondria with nodules and swollen endings, interposing between and passing through the fenestrated layered rER, were demonstrated in the functional cells. Oblique and cross-sections of both the rER system and tubular mitochondria showed orthodox configurations similar to those usually described in transmission electron microscopy (TEM) studies. Many finger-like projections constructing the cristae directing towards the inner mitochondrial chamber were observed, and external chamber extending into the tubular cristae was also demonstrated.

Recommended Citation

Iwai-Liao, Yasutomo; Higashi, Yoshikage; Ishikawa, Masakazu; and Hori, Hideaki (1992) "Scanning Electron Microscopy of Intracellular Organelles in the Young Odontoblasts of Rats," Scanning Microscopy: Vol. 6: No. 4, Article 19.
Available at: https://digitalcommons.usu.edu/microscopy/vol6/iss4/19