Title of Oral/Poster Presentation

Effects of Leachables and Extractables in Single Use Bioprocess Containment Systems on Chinese Hamster Ovary Cell Growth and Quality

Class

Article

Department

Biological and Irrigation Engineering

Faculty Mentor

Elizabeth Vargis

Presentation Type

Oral Presentation

Abstract

Recent studies have shown that certain living cells are sensitive to low levels of contaminants from polymer containers. These bioprocessing containers (BPC) are fundamental in the growth of cellular materials using single-use type bioprocessing. Many vaccines and antibiotics are created using BPCs.1 Irgafos 168 is a stabilizing agent that is used in many BPCs. It is used because it is highly resistant to hydrolysis and it protects the polymer.2 This study will look at the impact of irgafos 168 and determine the toxicity of the contaminant and how much irgafos 168 differents components of the BPC (tubing, bags, connections) will leach. Multiple bioreactor runs will be used to measure the amount of contaminants leached from the BPCs.

Start Date

4-9-2015 4:00 PM

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Apr 9th, 4:00 PM

Effects of Leachables and Extractables in Single Use Bioprocess Containment Systems on Chinese Hamster Ovary Cell Growth and Quality

Recent studies have shown that certain living cells are sensitive to low levels of contaminants from polymer containers. These bioprocessing containers (BPC) are fundamental in the growth of cellular materials using single-use type bioprocessing. Many vaccines and antibiotics are created using BPCs.1 Irgafos 168 is a stabilizing agent that is used in many BPCs. It is used because it is highly resistant to hydrolysis and it protects the polymer.2 This study will look at the impact of irgafos 168 and determine the toxicity of the contaminant and how much irgafos 168 differents components of the BPC (tubing, bags, connections) will leach. Multiple bioreactor runs will be used to measure the amount of contaminants leached from the BPCs.