Rapid prototyping of paper-based micro-device using wax and material printer for low-cost portable glucose assay
Class
Article
Graduation Year
2018
College
College of Engineering
Department
Biological Engineering Department
Faculty Mentor
Anhong Zhou
Presentation Type
Oral Presentation
Abstract
Here we present a simple and low-cost production method to generate paper-based microfluidic devices for portable glucose assay. The device consists of two parts: sample loading zone and bioassay zone. Black wax was printed on Whatman filter paper and baked at 150ºC for 2mins in order to build up hydrophobic boundary. DMP 2831 material printer was applied to print chromogenic substrate (3,3’,5,5’-Tetramethylbenzidine) enzyme mixture (glucose oxidase (GOx) and Horseradish peroxidase (HRP)) onto the bioasssay zone. Printing method provides more homogeneous coating than dropping on method. The ratio of GOx:HRP concentration is 20ug/ml:100ug/ml. The linear range is from 0.02mg/ml to 1mg/ml. Three different type of filter papers (Watman No.1, 4 and 5) were evaluated. The known and unknown concentration of glucose solutions were tested and compared by our device and other methods. The device is of low cost; fast testing; high stability and reliability. Our device provide a portable platform for blood and urea glucose concentration analysis.
Location
Room 208
Start Date
4-13-2017 10:30 AM
End Date
4-13-2017 11:45 AM
Rapid prototyping of paper-based micro-device using wax and material printer for low-cost portable glucose assay
Room 208
Here we present a simple and low-cost production method to generate paper-based microfluidic devices for portable glucose assay. The device consists of two parts: sample loading zone and bioassay zone. Black wax was printed on Whatman filter paper and baked at 150ºC for 2mins in order to build up hydrophobic boundary. DMP 2831 material printer was applied to print chromogenic substrate (3,3’,5,5’-Tetramethylbenzidine) enzyme mixture (glucose oxidase (GOx) and Horseradish peroxidase (HRP)) onto the bioasssay zone. Printing method provides more homogeneous coating than dropping on method. The ratio of GOx:HRP concentration is 20ug/ml:100ug/ml. The linear range is from 0.02mg/ml to 1mg/ml. Three different type of filter papers (Watman No.1, 4 and 5) were evaluated. The known and unknown concentration of glucose solutions were tested and compared by our device and other methods. The device is of low cost; fast testing; high stability and reliability. Our device provide a portable platform for blood and urea glucose concentration analysis.