Title of Oral/Poster Presentation

Impact of diet and microbiota composition on the phenotype of humanized mice using fecal microbiota transplant method.

Class

Article

Graduation Year

2018

College

College of Agriculture and Applied Sciences

Department

Animal, Dairy, and Veterinary Sciences Department

Faculty Mentor

Abby Benninghoff / Korry Hintze

Presentation Type

Poster Presentation

Abstract

Approximately 100 trillion bacteria, consisting of 400 to 500 different species, inhabit the human gastrointestinal tract. The composition of human gut microbiota can be affected by a variety of genetic and environmental factors, for example a poor diet. A Western dietary pattern is associated with dysbiosis and adverse health outcomes, including obesity and metabolic disorders. The primary objective of this study is to examine the contribution of gut microbiota from lean or obese human donors on development of metabolic syndrome and weight gain in recipient mice fed one of three basal diets: 1) the standard AIN93G, which is designed to promote rodent health; 2) the total Western diet (TWD), which promotes inflammation-associated colorectal carcinogenesis; and 3) a 45% high fat diet-induced obesity (DIO) diet, which promotes excessive weight gain and symptoms of metabolic syndrome. We hypothesized that mice receiving gut bacteria from obese humans would develop an obese phenotype with symptoms indicating metabolic disorder, which would be maintained by consumption of either the TWD or DIO diets. A 2x3 experiment design was used, where mice received fecal transfer from either lean or obese human donors and were fed one of the three diets listed above for 20 weeks. Prior to fecal transfer, the resident gut microbiome was depleted using an established antibiotic/antifugal oral dosing regimen. Endpoints assessed include body weight gain, body composition, food and energy intake, glucose tolerance and microbiota profiling of the human donor samples and fecal samples from recipient mice. Preliminary data for body weight gain, body composition and energy intake will be presented for this ongoing study at this Symposium.

Location

North Atrium

Start Date

4-13-2017 10:30 AM

End Date

4-13-2017 11:45 AM

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Apr 13th, 10:30 AM Apr 13th, 11:45 AM

Impact of diet and microbiota composition on the phenotype of humanized mice using fecal microbiota transplant method.

North Atrium

Approximately 100 trillion bacteria, consisting of 400 to 500 different species, inhabit the human gastrointestinal tract. The composition of human gut microbiota can be affected by a variety of genetic and environmental factors, for example a poor diet. A Western dietary pattern is associated with dysbiosis and adverse health outcomes, including obesity and metabolic disorders. The primary objective of this study is to examine the contribution of gut microbiota from lean or obese human donors on development of metabolic syndrome and weight gain in recipient mice fed one of three basal diets: 1) the standard AIN93G, which is designed to promote rodent health; 2) the total Western diet (TWD), which promotes inflammation-associated colorectal carcinogenesis; and 3) a 45% high fat diet-induced obesity (DIO) diet, which promotes excessive weight gain and symptoms of metabolic syndrome. We hypothesized that mice receiving gut bacteria from obese humans would develop an obese phenotype with symptoms indicating metabolic disorder, which would be maintained by consumption of either the TWD or DIO diets. A 2x3 experiment design was used, where mice received fecal transfer from either lean or obese human donors and were fed one of the three diets listed above for 20 weeks. Prior to fecal transfer, the resident gut microbiome was depleted using an established antibiotic/antifugal oral dosing regimen. Endpoints assessed include body weight gain, body composition, food and energy intake, glucose tolerance and microbiota profiling of the human donor samples and fecal samples from recipient mice. Preliminary data for body weight gain, body composition and energy intake will be presented for this ongoing study at this Symposium.