Title of Oral/Poster Presentation

Impact of the total Western diet and supplementation on inflammatory and immune response biomarkers

Class

Article

College

College of Agriculture and Applied Sciences

Faculty Mentor

Abby Benninghoff

Presentation Type

Oral Presentation

Abstract

Americans with leaky gut, or increased intestinal permeability, often develop chronic inflammation of the colon, which is considered a key driver in the development of diseases such as colorectal cancer (CRC) and inflammatory bowel disease (IBD). Immune response biomarkers such as tumor necrosis factor (TNF), ionized calcium-binding adapter molecule 1 (IBA1), cluster of differentiation 68 (CD68), and paired box 5 protein (PAX5) are a few of the contributors to this inflammation. Consumption of a poor diet, high in processed foods and low in fruits and vegetables, is another notable risk factor for CRC. Diet modification with functional foods containing health-promoting bioactive components or key micronutrients represents a safe and cost-effective strategy to decrease the incidence of cancer and/or delay the onset of the disease. Previously, our group assessed the impact of several dietary interventions in a mouse model of inflammation-associated CRC which included supplementation with black raspberries. The objective of the present work is to examine the impact of these dietary interventions, specifically black raspberries, on expression of molecular biomarkers of gut inflammation. The hypothesis to be tested in this study is that supplementation of a Western type diet with black raspberries will suppress expression of inflammatory and immune response biomarkers. Colon tissues were obtained from the aforementioned studies and processed for histopathology assessment. These tissues will be subject to immunohistochemistry for detection of TNF, IBA1, CD68, Ki67, and PAX5. Preliminary results of this ongoing work will be presented for the first time at this symposium.

Location

Room 101

Start Date

4-12-2018 3:00 PM

End Date

4-12-2018 4:15 PM

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Apr 12th, 3:00 PM Apr 12th, 4:15 PM

Impact of the total Western diet and supplementation on inflammatory and immune response biomarkers

Room 101

Americans with leaky gut, or increased intestinal permeability, often develop chronic inflammation of the colon, which is considered a key driver in the development of diseases such as colorectal cancer (CRC) and inflammatory bowel disease (IBD). Immune response biomarkers such as tumor necrosis factor (TNF), ionized calcium-binding adapter molecule 1 (IBA1), cluster of differentiation 68 (CD68), and paired box 5 protein (PAX5) are a few of the contributors to this inflammation. Consumption of a poor diet, high in processed foods and low in fruits and vegetables, is another notable risk factor for CRC. Diet modification with functional foods containing health-promoting bioactive components or key micronutrients represents a safe and cost-effective strategy to decrease the incidence of cancer and/or delay the onset of the disease. Previously, our group assessed the impact of several dietary interventions in a mouse model of inflammation-associated CRC which included supplementation with black raspberries. The objective of the present work is to examine the impact of these dietary interventions, specifically black raspberries, on expression of molecular biomarkers of gut inflammation. The hypothesis to be tested in this study is that supplementation of a Western type diet with black raspberries will suppress expression of inflammatory and immune response biomarkers. Colon tissues were obtained from the aforementioned studies and processed for histopathology assessment. These tissues will be subject to immunohistochemistry for detection of TNF, IBA1, CD68, Ki67, and PAX5. Preliminary results of this ongoing work will be presented for the first time at this symposium.