Title of Oral/Poster Presentation

Production, Purification, and Characterization of Recombinant Minor Ampullate Silk Proteins

Class

Article

College

College of Engineering

Faculty Mentor

Randolph Lewis

Presentation Type

Poster Presentation

Abstract

Spider silks have long been recognized for their impressive combinations of strength and elasticity. This project focuses on minor ampullate silk, one of the lesser studied of the 7 silks utilized in orb-weaving spiders. With a tensile strength similar to dragline silk, its properties mimic Kevlar and the human tendon. Due to an inability to farm spiders for their silk, producing these proteins synthetically is necessary to harness the properties of one of nature's most impressive materials. Using Escherichia coli as an expression system, this project focuses on the production, purification, and characterization of synthetic minor ampullate silk proteins.

Location

The South Atrium

Start Date

4-12-2018 10:30 AM

End Date

4-12-2018 11:45 AM

This document is currently not available here.

Share

COinS
 
Apr 12th, 10:30 AM Apr 12th, 11:45 AM

Production, Purification, and Characterization of Recombinant Minor Ampullate Silk Proteins

The South Atrium

Spider silks have long been recognized for their impressive combinations of strength and elasticity. This project focuses on minor ampullate silk, one of the lesser studied of the 7 silks utilized in orb-weaving spiders. With a tensile strength similar to dragline silk, its properties mimic Kevlar and the human tendon. Due to an inability to farm spiders for their silk, producing these proteins synthetically is necessary to harness the properties of one of nature's most impressive materials. Using Escherichia coli as an expression system, this project focuses on the production, purification, and characterization of synthetic minor ampullate silk proteins.