Production, Purification, and Characterization of Recombinant Minor Ampullate Silk Proteins
Class
Article
College
College of Engineering
Faculty Mentor
Randolph Lewis
Presentation Type
Poster Presentation
Abstract
Spider silks have long been recognized for their impressive combinations of strength and elasticity. This project focuses on minor ampullate silk, one of the lesser studied of the 7 silks utilized in orb-weaving spiders. With a tensile strength similar to dragline silk, its properties mimic Kevlar and the human tendon. Due to an inability to farm spiders for their silk, producing these proteins synthetically is necessary to harness the properties of one of nature's most impressive materials. Using Escherichia coli as an expression system, this project focuses on the production, purification, and characterization of synthetic minor ampullate silk proteins.
Location
The South Atrium
Start Date
4-12-2018 10:30 AM
End Date
4-12-2018 11:45 AM
Production, Purification, and Characterization of Recombinant Minor Ampullate Silk Proteins
The South Atrium
Spider silks have long been recognized for their impressive combinations of strength and elasticity. This project focuses on minor ampullate silk, one of the lesser studied of the 7 silks utilized in orb-weaving spiders. With a tensile strength similar to dragline silk, its properties mimic Kevlar and the human tendon. Due to an inability to farm spiders for their silk, producing these proteins synthetically is necessary to harness the properties of one of nature's most impressive materials. Using Escherichia coli as an expression system, this project focuses on the production, purification, and characterization of synthetic minor ampullate silk proteins.