Title of Oral/Poster Presentation

Micropropagation of Ceanothus velutinus: Stage I

Class

Article

College

College of Agriculture and Applied Sciences

Faculty Mentor

Youping Sun

Presentation Type

Poster Presentation

Abstract

Plant tissue culture is the growth of plant organs or tissues in aseptic culture where the environment as well as nutrient and hormone levels are tightly controlled. Tissue culture, or micropropagation, is useful for propagating plants that are difficult or slow to propagate with other approaches. Micropropagation consists of four stages. stage I: establishment of explants, stage II: shoot multiplication, stage III root formation, and stage IV: acclimatization. The goal of stage I is to place an explant into aseptic culture which provides an environment that promotes shoot production, while avoiding contamination. The objective of this experiment is to establish a clean culture for the micropropagation of Ceanothus velutinus, a Utah native plant with significance in the green industry. Cuttings of Ceanothus velutinus were collected from a research greenhouse, wrapped with wet paper towels, placed in Ziplock bags, and brought into the lab. Leaves were peeled off and cuttings were disinfected using 10% bleach and 70% ethanol and established on Murashige and Skoog (MS) medium with 0.1 mg/L benzyl adenine at 25° C with a 16-hour photoperiod under cool white fluorescent lamps. One month later, 63% had been contaminated, 27% had lived, and 10% had browned. These live explants are still under cultivation to establish an efficient mass propagation protocol for Utah native plants.

Location

The South Atrium

Start Date

4-12-2018 10:30 AM

End Date

4-12-2018 11:45 AM

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Apr 12th, 10:30 AM Apr 12th, 11:45 AM

Micropropagation of Ceanothus velutinus: Stage I

The South Atrium

Plant tissue culture is the growth of plant organs or tissues in aseptic culture where the environment as well as nutrient and hormone levels are tightly controlled. Tissue culture, or micropropagation, is useful for propagating plants that are difficult or slow to propagate with other approaches. Micropropagation consists of four stages. stage I: establishment of explants, stage II: shoot multiplication, stage III root formation, and stage IV: acclimatization. The goal of stage I is to place an explant into aseptic culture which provides an environment that promotes shoot production, while avoiding contamination. The objective of this experiment is to establish a clean culture for the micropropagation of Ceanothus velutinus, a Utah native plant with significance in the green industry. Cuttings of Ceanothus velutinus were collected from a research greenhouse, wrapped with wet paper towels, placed in Ziplock bags, and brought into the lab. Leaves were peeled off and cuttings were disinfected using 10% bleach and 70% ethanol and established on Murashige and Skoog (MS) medium with 0.1 mg/L benzyl adenine at 25° C with a 16-hour photoperiod under cool white fluorescent lamps. One month later, 63% had been contaminated, 27% had lived, and 10% had browned. These live explants are still under cultivation to establish an efficient mass propagation protocol for Utah native plants.