Title of Oral/Poster Presentation

Developing a Large Animal Tendinothapy Model for Testing TIMP-3 Gene Therapy

Presenter Information

Kasie Laverty, Utah State University

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Class

Article

College

College of Science

Presentation Type

Oral Presentation

Abstract

In performance horses, injury to the superficial digital flexor tendon (SDTF) is common (Habermehl et al., 2019). This injury is brought on suddenly, and even with healing, it is often reinjured during the life of the horse. There have been various studies using therapies to help improve the way we treat tendon injuries and to reduce the risk of reinjury (Taylor, 2013). In this model, we will use TIMP-3, a protein that has been used to prevent degradation and reduce scar tissue build up (Lim et al., 2010 ), which we have previously demonstrated in Dr. Mason's lab using TIMP-3 in the stifle joint in an ovine model. This injury was created surgically, and the TIMP-3 treatment was paired with exercise (Hill et al., 2017). The current study will evaluate the effects of water thermodynamic damage to tendons, followed by an injection of TIMP-3 a week after injury. We will inject TIMP-3 directly into the mid-metacarpal region where the tendon is located. We will first use this in an in vitro model and will then use large animal models for in vivo measurements (Habermehl et al., 2019). We expect to have results showing that the introduction of TIMP-3 into the tendon lesion will help improve the healing process of the tendon and prevent further damage. We anticipate this leading to a longer duration of health and the ability of the horse to return to work.

Start Date

4-8-2020 2:00 PM

End Date

4-8-2020 3:00 PM

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Apr 8th, 2:00 PM Apr 8th, 3:00 PM

Developing a Large Animal Tendinothapy Model for Testing TIMP-3 Gene Therapy

In performance horses, injury to the superficial digital flexor tendon (SDTF) is common (Habermehl et al., 2019). This injury is brought on suddenly, and even with healing, it is often reinjured during the life of the horse. There have been various studies using therapies to help improve the way we treat tendon injuries and to reduce the risk of reinjury (Taylor, 2013). In this model, we will use TIMP-3, a protein that has been used to prevent degradation and reduce scar tissue build up (Lim et al., 2010 ), which we have previously demonstrated in Dr. Mason's lab using TIMP-3 in the stifle joint in an ovine model. This injury was created surgically, and the TIMP-3 treatment was paired with exercise (Hill et al., 2017). The current study will evaluate the effects of water thermodynamic damage to tendons, followed by an injection of TIMP-3 a week after injury. We will inject TIMP-3 directly into the mid-metacarpal region where the tendon is located. We will first use this in an in vitro model and will then use large animal models for in vivo measurements (Habermehl et al., 2019). We expect to have results showing that the introduction of TIMP-3 into the tendon lesion will help improve the healing process of the tendon and prevent further damage. We anticipate this leading to a longer duration of health and the ability of the horse to return to work.