Event Title

Extremophile Dormancy: Using Targeted Metagenomics to Identify Microbial Community Composition in Hypersaline and Freshwater Lakes

Presenter Information

Joshua Vert

Location

ECC 216

Event Website

http://water.usu.edu/

Start Date

4-9-2013 1:30 PM

End Date

4-9-2013 1:50 PM

Description

From acid seeps and deep-sea thermal vents to glacial ice and hypersaline lakes, extreme environments contain relatively simplified communities consisting of organisms that evolved to survive and thrive under adverse environmental conditions. Although microbial dormancy is thought to be a survival mechanism for microbes in extreme environments, it is unclear if microbial dormancy rates differ between extreme and neutral environments. Using salinity, pH, and dissolved oxygen levels as measurements of ‘extreme,’ we hypothesized that bacterial and archaeal taxa in hypersaline lake communities will exhibit lower levels dormancy than bacterial and archaeal communities in geologically similar freshwater lake controls. For our study, we defined dormancy as the difference between DNA-based communities (i.e., all microorganisms present in the community) and RNA-based communities (only the microbes which are active) and used targeted metagenomics to analyze the 16S rDNA and rRNA extracted from five hypersaline and freshwater lakes across the western United States. We also hypothesized that dormancy of the communities will be contingent on the extremity of the environment. As hypothesized, DNA-based and RNA-based bacterial communities were more similar in hypersaline (F =1.121; P =0.09) than freshwater lakes (F =19.605; P

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Apr 9th, 1:30 PM Apr 9th, 1:50 PM

Extremophile Dormancy: Using Targeted Metagenomics to Identify Microbial Community Composition in Hypersaline and Freshwater Lakes

ECC 216

From acid seeps and deep-sea thermal vents to glacial ice and hypersaline lakes, extreme environments contain relatively simplified communities consisting of organisms that evolved to survive and thrive under adverse environmental conditions. Although microbial dormancy is thought to be a survival mechanism for microbes in extreme environments, it is unclear if microbial dormancy rates differ between extreme and neutral environments. Using salinity, pH, and dissolved oxygen levels as measurements of ‘extreme,’ we hypothesized that bacterial and archaeal taxa in hypersaline lake communities will exhibit lower levels dormancy than bacterial and archaeal communities in geologically similar freshwater lake controls. For our study, we defined dormancy as the difference between DNA-based communities (i.e., all microorganisms present in the community) and RNA-based communities (only the microbes which are active) and used targeted metagenomics to analyze the 16S rDNA and rRNA extracted from five hypersaline and freshwater lakes across the western United States. We also hypothesized that dormancy of the communities will be contingent on the extremity of the environment. As hypothesized, DNA-based and RNA-based bacterial communities were more similar in hypersaline (F =1.121; P =0.09) than freshwater lakes (F =19.605; P

https://digitalcommons.usu.edu/runoff/2013/AllAbstracts/25