Location
Room # EB302
Start Date
5-6-2019 11:10 AM
Description
Increased sensor sensitivity is important for enabling detection of low concentrations of target analyte. Being able to quickly and accurately detect low concentrations of proteins at point-of-care allows for results to be analyzed more easily and effectively. Vertically-aligned carbon nanotubes (VACNTs) were patterned into interdigitated electrodes (IDEs) and then functionalized with the representative protein streptavidin that was functionalized on the VACNT surfaces by covalent bonding (with EDC/NHS). Further detection was observed by binding biotin to the streptavidin. Fluorescence microscopy enabled the optimization of protein loading on VACNTs. Cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize the electrodes and monitor the associated changes with the addition of streptavidin and biotin. A unique change in impedance is observed, which allows for monitoring the quantity of target analyte bound to the VACNT electrode surface with an observed 14x increase in resistance.
3D Interdigitated Electrodes for Point-of-Care Electochemical Biosensing
Room # EB302
Increased sensor sensitivity is important for enabling detection of low concentrations of target analyte. Being able to quickly and accurately detect low concentrations of proteins at point-of-care allows for results to be analyzed more easily and effectively. Vertically-aligned carbon nanotubes (VACNTs) were patterned into interdigitated electrodes (IDEs) and then functionalized with the representative protein streptavidin that was functionalized on the VACNT surfaces by covalent bonding (with EDC/NHS). Further detection was observed by binding biotin to the streptavidin. Fluorescence microscopy enabled the optimization of protein loading on VACNTs. Cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize the electrodes and monitor the associated changes with the addition of streptavidin and biotin. A unique change in impedance is observed, which allows for monitoring the quantity of target analyte bound to the VACNT electrode surface with an observed 14x increase in resistance.
Comments
Session 5