Isolation of 11 Polymorphic Tri- and Tetranucleotide Microsatellite Loci in a North American Sedge (Carex scoparia: Cyperaceae) and Cross-Species Amplification in Three Additional Carex Species

Authors

Andrew L. Hipp
Karin M. Kettenring, Utah State UniversityFollow
Kevin A. Feldheim
Jaime A. Weber

Document Type

Article

Journal/Book Title/Conference

Molecular Ecology Resources

Volume

9

Issue

2

Publisher

Wiley-Blackwell

Publication Date

2009

Keywords

Carex subgenus Vignea, chromosome diversity, Cyperaceae, microsatellite enrichment, sedges

First Page

625

Last Page

627

Abstract

We report on the isolation and evaluation of 11 microsatellites from a widespread eastern North American wetland sedge, Carex scoparia. Loci exhibit 3–9 alleles over five populations and significant F_IS (0.204–0.717) in most populations. All primers cross-amplify in at least two other species, and 10 cross-amplify in the more distantly related C. stipata. These markers will be used to examine population genetics and patterns of chromosomal diversification in this ecologically important sedge species and its relatives.

Comments

Originally published by Wiley-Blackwell. Publisher's PDF and HTML fulltext available through remote link. Karin Kettenring was working for the Smithsonian Environmental Research Center at the time this article was published.

Recommended Citation

Hipp, A. L., Kettenring, K. M., Feldheim, K. A. and Weber, J. A. (2009), Isolation of 11 polymorphic tri- and tetranucleotide microsatellite loci in a North American sedge (Carex scoparia: Cyperaceae) and cross-species amplification in three additional Carex species. Molecular Ecology Resources, 9: 625–627.