In Vitro Propagation of the Endangered Virginia Roundleaf Birch (Betula uber [Ashe] Fern.) Using Dormant Buds
Dormant buds collected from greenhouse and field-grown trees of Betula uber (Ashe) Fern., the endangered Virginia round-leaf birch, were used for micropropagation. A nutrient medium containing the mineral salts of Murashige and Skoog (1962) supplemented with organic nutrients and growth regulators supported bud break, elongation, and limited multiplication. Shoots were 2-3 cm in height with 3-4 leaves in four weeks. They developed roots in vivo in 4-6 weeks in peat pellets under high humidity conditions with 16 h photoperiod at a temperature of 20°C.
Sharik, Terry L.; Vijayakumar, N. K.; and Feret, Peter P., "In Vitro Propagation of the Endangered Virginia Roundleaf Birch (Betula uber [Ashe] Fern.) Using Dormant Buds" (1990). Wildland Resources Faculty Publications. Paper 1785.