Inhibitory Effect of Aroclor 1254 on Aflatoxin-Initiated Carcinogenesis in Rainbow Trout and Mutagenesis Using a Salmonella/Trout Hepatic Activation System

Document Type

Article

Journal/Book Title/Conference

Aquatic Toxicology

Volume

3

Issue

3

Publisher

Elsevier

Publication Date

1983

First Page

229

Last Page

238

Abstract

Duplicate lots of 140 rainbow trout (Salmo gairdneri) were fed either control diet (CD) or 100 ppm Aroclor 1254 (a polychlorinated biphenyl—PCB) for 3 mth followed by initiation of liver carcinomas with 20 ppb dietary aflatoxin B1 (AFB1) for 2 wk. At 8 and 12 mth after AFB1 treatment, fish were sampled and tumor incidence determined. Trout that were prefed PCB showed a 45% inhibition in tumor incidence at 12 mth, when compared to those prefed CD. Throughout the experiment fish were sampled to determine the time relationship of PCB bioaccumulation. A rapid uptake of PCB into total body fat was seen, with concentration of 594 ppm at the time of AFB1 exposure. Liver benzo[a]pyrehe monooxygenase (B[a]PM) activity was also induced at the time AFB1 exposure began. The Ames mutagenesis assay was used to determine the effects of in vitro Aroclor 1254 on AFB1-induced mutagenesis using a trout liver preparation. A PCB dose-related inhibition was observed with a 67% inhibition at the highest dose tested (500 μg Aroclor 1254/plate). Proposed mechanisms of the inhibition of AFB1-induced carcinogenesis/mutagenesis are discussed.

Comments

Originally published by Elsevier. Publisher's PDF available through remote link.

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