In two experiments the computation of sarcomere length from laser diffraction patterns was tested for accuracy against phase-contrast microscopy . Particular attention was paid to methodological factors such as sampling location and computation formulae. Correlation coefficients between the laser diffraction technique and the microscopical method were high (r= 0.96) in both experiments. However, when computed from a simplified formula, the sarcomere length values, determined by laser diffraction patterns tended to be approximately 0. 10 lJm lower. It is recommended to use the correct formula in computing the laser diffraction data and to investigate a limited number of fibres (3) in a sufficiently large number (5) of randomly distributed samples.
Koolmees, Peter A.; Korteknie, Frank; and Smulders, Frans J.M.
"Accuracy and Utility of Sarcomere Length Assessment by Laser Diffraction,"
1, Article 9.
Available at: http://digitalcommons.usu.edu/foodmicrostructure/vol5/iss1/9