Class
Article
College
College of Agriculture and Applied Sciences
Department
Plants, Soils, and Climate Department
Faculty Mentor
Amita Kaundal
Presentation Type
Poster Presentation
Abstract
Salinity is one of the major environmental stresses that affect crop growth and productivity. Almond crop has a great potential for Utah as a cash crop and salinity is one of the stresses affecting its growth and yield. Although almond trees are sensitive to salt stress, salinity-tolerant rootstocks can play a significant role in sustaining crop production in semiarid areas. Salinity tolerance has a complex mechanism with many genes acting in different pathways. Understanding the role of potential candidate genes is an important aspect of genomics assisted improvement of stress tolerance. In this study, functional complementation of the Prunus persica Salt Overly Sensitive 2 (ppsos2) gene from the almond rootstock ‘Nemaguard’ was done. Two transgenic lines of ppsos2 were developed into the mutant atsos2 background using constitutive (PpSOS2.OE3.5) and endogenous (PpSOS2NP.2.7) promoters. Both transgenic lines showed significantly higher survival rates and dry weight than atsos2 under 90 mM NaCl treatment. The atsos2 mutant inhibited growth of primary and lateral roots when treated with 50 mM NaCl. The root growth inhibition was restored by ppsos2 complementation. Both transgenic lines showed a significant decrease in electrolyte leak percentage compared to atsos2 when treated with 50 mM NaCl. The electrolyte leakage percentage in transgenic lines was the same as in wild-type plants after treatment. The expression of ppsos2 was induced in both transgenic lines under 90 mM NaCl salt treatment. Expression analysis of six K+-rectifying channel genes revealed differential expression of atakt1, atakt2, and atchx14 genes in transgenic compared to the atsos2 mutant 24 h after the 50 mM NaCl treatment. These observations suggest that ppsos2 modulates and restores salt tolerance in atsos2 and that SOS pathway is conserved in Prunus. Presentation Time: Thursday, 2-3 p.m.
Location
Logan, UT
Start Date
4-12-2021 12:00 AM
Included in
Climate Commons, Plant Sciences Commons, Soil Science Commons
Transgenic Expression of Prunus persica Salt Overly Sensitive 2 (ppsos2) in atsos2 Mutant Imparts Salt Tolerance in Arabidopsis
Logan, UT
Salinity is one of the major environmental stresses that affect crop growth and productivity. Almond crop has a great potential for Utah as a cash crop and salinity is one of the stresses affecting its growth and yield. Although almond trees are sensitive to salt stress, salinity-tolerant rootstocks can play a significant role in sustaining crop production in semiarid areas. Salinity tolerance has a complex mechanism with many genes acting in different pathways. Understanding the role of potential candidate genes is an important aspect of genomics assisted improvement of stress tolerance. In this study, functional complementation of the Prunus persica Salt Overly Sensitive 2 (ppsos2) gene from the almond rootstock ‘Nemaguard’ was done. Two transgenic lines of ppsos2 were developed into the mutant atsos2 background using constitutive (PpSOS2.OE3.5) and endogenous (PpSOS2NP.2.7) promoters. Both transgenic lines showed significantly higher survival rates and dry weight than atsos2 under 90 mM NaCl treatment. The atsos2 mutant inhibited growth of primary and lateral roots when treated with 50 mM NaCl. The root growth inhibition was restored by ppsos2 complementation. Both transgenic lines showed a significant decrease in electrolyte leak percentage compared to atsos2 when treated with 50 mM NaCl. The electrolyte leakage percentage in transgenic lines was the same as in wild-type plants after treatment. The expression of ppsos2 was induced in both transgenic lines under 90 mM NaCl salt treatment. Expression analysis of six K+-rectifying channel genes revealed differential expression of atakt1, atakt2, and atchx14 genes in transgenic compared to the atsos2 mutant 24 h after the 50 mM NaCl treatment. These observations suggest that ppsos2 modulates and restores salt tolerance in atsos2 and that SOS pathway is conserved in Prunus. Presentation Time: Thursday, 2-3 p.m.