Purification of Bovine Sperm Membrane-Proteins Involved in Sperm-Oocyte Interactions: Adhesion, Fusion, and Activation
Society for the Study of Reproduction
At fertilization interactions between sperm and oocyte proteins are pivotal to oocyte activation, fusion and embryo development.The proteins involved in bovine activation have not yet been isolated, identified or characterized. A working hypothesis for the interaction between the sperm and the oocyte is that protein ligands on the inner acrosomal membrane of the sperm bind to protein receptors on the oocyte vitelline membrane.Previously, our laboratory utilized in situ fluorescent labeling of proteins and 2-D gel electrophoresis to identify candidate sperm protein ligands involved in bovine sperm-oocyte interactions.To further this investigation ion exchange chromatography was used to isolate and quantify the proteins of interest derived from the 2-D gel studies.
Bayles, A., Sessions, B.R., Aston, K.I., Brinkerhoff, J., Chen, D., Weimer, B. and White, K.L. 2007. Purification of Bovine Sperm Membrane-Proteins Involved in Sperm-Oocyte Interactions: Adhesion, Fusion, and Activation. Biol. Reprod. 57:.