Title
Anti-humancytomegalovirus activity and toxicity of sulfonated anthraquinones and anthraquinonederivatives
Document Type
Article
Journal/Book Title/Conference
Anti-humancytomegalovirus activity and toxicity of sulfonated anthraquinones and anthraquinonederivatives
Volume
28
Publication Date
1995
First Page
317
Last Page
329
Abstract
Sulfonated anthraquinones and other anthraquinone derivatives were evaluated for anti-human cytomegalovirus (HCMV) activity, cytotoxicity and genotoxicity. Acid blues 40 and 129, acid black 48, alizarin violet R and reactive blue 2 were the most active compounds having selective indices of greater than 30 and ECs0 values of 4-30 p.M. When tested against a clinical isolate, the 4 compounds were 2- to 5-fold less active. The antiviral activity was distinctly separate from the virucidal activity (> 1000 /zM). The compounds were weakly toxic to either log phase or stationary cells in most of the following cytotoxicity assays: neutral red uptake assay, lactic acid dehydrogenase assay, trypan blue exclusion assay and radiolabeled macromolecular precursor uptake assays. Using a genotoxicity assay, the comet assay, only reactive blue 2 and acid black 48 were found to cause DNA strand breakage. This occurred at concentrations of 30 and 170 /zM, respectively. These results suggest that these compounds could be a prototype for synthesizing even more effective HCMV-inhibitory anthraquinone derivatives.
Recommended Citation
Barnard, D.L., D.W. Fairbairn, K.L. O'Neill, T.L. Gage and R.W. Sidwell. 1995. Anti-human cytomegalovirus activity and toxicity of sulfonated anthraquinones and anthraquinone derivatives. Antiviral Res. 28: 317-329. PMID: 8669891
Comments
Antiviral Res. 28: 317-329. PMID: 8669891