Profiling of small non-coding RNA in bovine somatic cell nuclear transfer embryos during early development through the maternal-to-embryonic transition

Creators

Abby D. Benninghoff, Utah State UniversityFollow
Jocelyn Cuthbert, Utah State UniversityFollow

Description

Supplementary File 1. Read lengths and annotation summaries of sncRNA: Microsoft Excel document with information from small RNA sequencing on the aligned, annotated reads.

Supplementary File 2. Normalized and variance-stabilized values for annotated sncRNAs: Microsoft Excel document with normalized and variance-stabilized reads generated by RNA sequencing of small non-coding RNA from cattle embryos.

Supplementary File 3. Results of DESeq2 analyses for miRNAs, tRNA fragments and piRNAs: Microsoft Excel document with results of DESeq2 differential expression analysis comparing scNT vs IVF embryos at each developmental stage and sequential comparisons of developmental stage for both IVF and scNT embryos.

Supplementary File 4. TargetScan prediction of mRNA targets for differentially expressed miRNAs: Microsoft Excel document providing results of TargetScan prediction of mRNA targets for differentially expressed miRNAs.

Supplementary File 5. Results of Metascape ontology analyses for predicted mRNA targets of differentially expressed miRNAs: Microsoft Excel document providing results of Metascape ontology analysis for biological processes associated with differentially expressed miRNAs.

Supplementary File 6 . Mapping of piRNAs to transposable elements: Microsoft Excel document providing results of ProTrac mapping of putative piRNAs to transposable elements in the bovine genome.

Author ORCID Identifier

Abby D. Benninghoff https://orcid.org/0000-0002-7993-0117

Jocelyn Cuthbert https://orcid.org/0000-0002-9985-0392

OCLC

1153396381

Document Type

Dataset

DCMI Type

Dataset

File Format

.xlsx, .docx

Publication Date

4-23-2020

Funder

USDA, National Institute of Food and Agriculture (NIFA)

Publisher

Utah State University

Award Number

USDA, National Institute of Food and Agriculture (NIFA) 2018-67011-28087

Award Title

DNA Methylation and MicroRNA Expression in Bovine Somatic Embryos

Referenced by

This data set is supporting material for a dissertation and a forthcoming journal article.

Language

eng

Code Lists

See the applicable DC form file for the code list.

Comments

The DC_form_file_3-x files contain the README information and code list for each Supplementary file, with "x" corresponding with the applicable file.

Disciplines

Developmental Biology

License

This work is licensed under a Creative Commons Attribution 4.0 License.

Identifier

https://doi.org/10.26078/8jjx-1v90

Recommended Citation

Benninghoff, A. D., & Cuthbert, J. (2020). Profiling of small non-coding RNA in bovine somatic cell nuclear transfer embryos during early development through the maternal-to-embryonic transition. Utah State University. https://doi.org/10.26078/8JJX-1V90