Supplementary File 1. Read lengths and annotation summaries of sncRNA: Microsoft Excel document with information from small RNA sequencing on the aligned, annotated reads.
Supplementary File 2. Normalized and variance-stabilized values for annotated sncRNAs: Microsoft Excel document with normalized and variance-stabilized reads generated by RNA sequencing of small non-coding RNA from cattle embryos.
Supplementary File 3. Results of DESeq2 analyses for miRNAs, tRNA fragments and piRNAs: Microsoft Excel document with results of DESeq2 differential expression analysis comparing scNT vs IVF embryos at each developmental stage and sequential comparisons of developmental stage for both IVF and scNT embryos.
Supplementary File 4. TargetScan prediction of mRNA targets for differentially expressed miRNAs: Microsoft Excel document providing results of TargetScan prediction of mRNA targets for differentially expressed miRNAs.
Supplementary File 5. Results of Metascape ontology analyses for predicted mRNA targets of differentially expressed miRNAs: Microsoft Excel document providing results of Metascape ontology analysis for biological processes associated with differentially expressed miRNAs.
Supplementary File 6 . Mapping of piRNAs to transposable elements: Microsoft Excel document providing results of ProTrac mapping of putative piRNAs to transposable elements in the bovine genome.
Author ORCID Identifier
Abby D. Benninghoff https://orcid.org/0000-0002-7993-0117
Jocelyn Cuthbert https://orcid.org/0000-0002-9985-0392
USDA, National Institute of Food and Agriculture (NIFA)
Utah State University
USDA, National Institute of Food and Agriculture (NIFA) 2018-67011-28087
DNA Methylation and MicroRNA Expression in Bovine Somatic Embryos
This data set is supporting material for a dissertation and a forthcoming journal article.
See the applicable DC form file for the code list.
This work is licensed under a Creative Commons Attribution 4.0 License.
Benninghoff, A. D., & Cuthbert, J. (2020). Profiling of small non-coding RNA in bovine somatic cell nuclear transfer embryos during early development through the maternal-to-embryonic transition. Utah State University. https://doi.org/10.26078/8JJX-1V90
Additional FilesSupplementary_File_1.xlsx (58 kB)
DC_form_file_3-1.docx (16 kB)
Supplementary_File_2.xlsx (6148 kB)
DC_form_file_3-2.docx (17 kB)
Supplementary_File_3.xlsx (8257 kB)
DC_form_file_3-3.docx (18 kB)
Supplementary_File_4.xlsx (2932 kB)
DC_form_file_3-4.docx (17 kB)
Supplementary_File_5.xlsx (262 kB)
DC_form_file_3-5.docx (18 kB)
Supplementary_File_6.xlsx (6575 kB)
DC_form_file_3-6.docx (16 kB)