Genetic variation at the species and population levels in the Rocky Mountain ridged mussel (Gonidea angulata) – Supplementary Material

Creators

James A. Walton, Utah State UniversityFollow
Karen E. Mock, Utah State UniversityFollow
Steven F. R. Brownlee, Simon Fraser UniversityFollow
Jon H. Mageroy, Norwegian Institute for Nature Research (NINA)Follow
Greg Wilson, British Columbia Ministry of Environment and Climate Change StrategyFollow
Ian R. Walker, University of British Columbia OkanaganFollow

Description

Freshwater mussels in western North America are threatened by water diversions, climate change, loss of required host fish, and other factors, and have experienced marked decline in the past several decades. All four of the primary lineages (potentially species) of freshwater mussels in the western U.S. and Canada are widespread and have somewhat generalist host fish requirements. Of these lineages, perhaps the most poorly understood and of greatest conservation concern is Gonidea angulata (Rocky Mountain ridged mussel). Gonidea is a monotypic genus occurring only in the western continental U.S. and southern Canada. Here we describe the patterns of genetic variation across the species range, including several populations in the Okanagan Valley at the northern edge of the range. We detected only ten mitochondrial cytochrome oxidase I haplotypes, three of which are commonly found across major hydrologic basins, and the remainder of which were basin-specific variants. Haplotypes differed by a maximum of 5 of 537 nucleotides. New microsatellite loci were developed for G. angulata as a part of this study. Data from these microsatellite loci indicated that the population in the Chehalis River, Washington, was distinct from other locations, and that the Okanagan lake population was somewhat diverged from the remaining populations in the Columbia River and Klamath Lake. Only low levels of inbreeding were detected, in contrast to previous findings in Margartifera falcata, suggesting that hermaphroditism is not common. The population with the least diversity, according to microsatellite data, was the northern-most known population in Okanagan Lake. We discuss the biogeographic and conservation implications of our findings and provide supplementary material of our research including sequencing data and narrative of microsatellite development.

Author ORCID Identifier

James A Walton https://orcid.org/0000-0003-3211-5083

Steven F. R. Brownlee https://orcid.org/0000-0003-0598-3061

Ian R. Walker https://orcid.org/0000-0002-2385-6165

OCLC

1236209375

Document Type

Dataset

DCMI Type

Dataset

File Format

.pdf, txt, sh, fasta, xlsx, fastq.gz

Viewing Instructions

Instructions to concatenate files for use included in the README.txt file

Publication Date

11-6-2020

Funder

British Columbia Ministry of Environment and Climate Change Strategy (Conservation Science Section)

Publisher

Utah State University

Methodology

Samples were collected from the Okanagan, Chehalis, Columbia, Klamath, and Sacramento River Basins using non-lethal collection techniques. Paired-end 'Shotgun' sequences were aligned, process, and optimized for multiplex conditions. Additional information can be found in the Microsatellite Development Narrative (Document S1)

Scientfic Names

Gonidea angulata

Language

eng

Code Lists

See Document_S1_Microsatellite_Development_Narrative.pdf for code list.

Comments

Description of files:

Document S1: Microsatellite Development Narrative

Documents S2-1 to S2-4: Raw sequence data divided into 4 parts. (See instructions for concatenation in the README.txt file)

Document S3: Screening Scripts

Document S4: Filtered Sequences

Document S5: Primer Design

Document S6: Microsatellite Genotype Data

Figure S1: Structure Harvester Results

Table S1: Sampling Locations (Generalized location data is provided. For more detailed location information contact the authors listed above.)

Table S2: Microsatellite Loci and Multiplexing

Table S3: COI Genbank Accessions

Disciplines

Natural Resources Management and Policy

License

This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.

Recommended Citation

Walton, J. A., Mock, K. E., Brownlee, S. F. R., Mageroy, J. H., Wilson, G., & Walker, I. R. (2020). Genetic variation at the species and population levels in the Rocky Mountain ridged mussel (Gonidea angulata) – Supplementary Material [Data set]. Utah State University. https://doi.org/10.26078/FGMQ-BM18

Checksum

019bfb1317bd4935a4196f661c62b6df