Freshwater mussels in western North America are threatened by water diversions, climate change, loss of required host fish, and other factors, and have experienced marked decline in the past several decades. All four of the primary lineages (potentially species) of freshwater mussels in the western U.S. and Canada are widespread and have somewhat generalist host fish requirements. Of these lineages, perhaps the most poorly understood and of greatest conservation concern is Gonidea angulata (Rocky Mountain ridged mussel). Gonidea is a monotypic genus occurring only in the western continental U.S. and southern Canada. Here we describe the patterns of genetic variation across the species range, including several populations in the Okanagan Valley at the northern edge of the range. We detected only ten mitochondrial cytochrome oxidase I haplotypes, three of which are commonly found across major hydrologic basins, and the remainder of which were basin-specific variants. Haplotypes differed by a maximum of 5 of 537 nucleotides. New microsatellite loci were developed for G. angulata as a part of this study. Data from these microsatellite loci indicated that the population in the Chehalis River, Washington, was distinct from other locations, and that the Okanagan lake population was somewhat diverged from the remaining populations in the Columbia River and Klamath Lake. Only low levels of inbreeding were detected, in contrast to previous findings in Margartifera falcata, suggesting that hermaphroditism is not common. The population with the least diversity, according to microsatellite data, was the northern-most known population in Okanagan Lake. We discuss the biogeographic and conservation implications of our findings and provide supplementary material of our research including sequencing data and narrative of microsatellite development.
.pdf, txt, sh, fasta, xlsx, fastq.gz
Instructions to concatenate files for use included in the README.txt file
British Columbia Ministry of Environment and Climate Change Strategy (Conservation Science Section)
Utah State University
Samples were collected from the Okanagan, Chehalis, Columbia, Klamath, and Sacramento River Basins using non-lethal collection techniques. Paired-end 'Shotgun' sequences were aligned, process, and optimized for multiplex conditions. Additional information can be found in the Microsatellite Development Narrative (Document S1)
See Document_S1_Microsatellite_Development_Narrative.pdf for code list.
Natural Resources Management and Policy
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 License.
Walton, J. A., Mock, K. E., Brownlee, S. F. R., Mageroy, J. H., Wilson, G., & Walker, I. R. (2020). Genetic variation at the species and population levels in the Rocky Mountain ridged mussel (Gonidea angulata) – Supplementary Material [Data set]. Utah State University. https://doi.org/10.26078/FGMQ-BM18
Additional FilesREADME.txt (6 kB)
Document_S1_Microsatellite_Development_Narrative.pdf (171 kB)
Document_S2-1_gonideaF_read1_aa_fastq.gz (2184713 kB)
Document_S2-2_gonideaF_read1_ab_fastq.gz (2206431 kB)
Document_S2-3_gonideaR_read2_aa_fastq.gz (2481653 kB)
Document_S2-4_gonideaR_read2_ab_fastq.gz (2536552 kB)
Document_S3_Screening_Scripts.sh (7 kB)
Document_S4_Filtered_Sequences.fasta (543 kB)
Document_S5_Primer_Design.xlsx (129 kB)
Document_S6_Microsatellite_Genotype_Data.xlsx (100 kB)
Figure_S1_Structure_Harvester_Results.pdf (11 kB)
Table_S1_Sampling_Locations.xlsx (12 kB)
Table_S2_ Microsatellite_Loci_and_Multiplexing.pdf (143 kB)
Table_S3_COI_GenBank_Accessions.pdf (131 kB)