Dynamic Fecal Microbiota Transfer from Mice with Western Diet-Induced Severe Colitis and Colon Tumorigenesis Did Not Exacerbate Colitis Symptoms or Colon Tumorigenesis in Recipient Mice Fed Either the AIN93G Diet or the Total Western Diet

Creators

Abby D. Benninghoff, Utah State UniversityFollow

Description

Consumption of the total Western diet (TWD) in mice has been shown to increase gut inflammation, promote colon tumorigenesis, and alter the fecal microbiome composition in mice as com-pared to mice fed a healthy diet, AIN93G (AIN). However, it is unclear whether the gut micro-biome contributes directly to colitis-associated CRC in this model. The objective of this study was to determine whether dynamic fecal microbiota transfer (FMT) from host mice fed either AIN or TWD basal diets would alter colitis symptoms or colitis-associated CRC in recipient mice, which were fed either AIN or TWD directly using a 2x2 factorial experiment design. Time-matched FMT from donor mice fed TWD did not significantly enhance symptoms of colitis, colon epithelial inflammation, mucosal injury, or colon tumor burden in recipient mice fed AIN diet. Conversely, FMT from AIN-fed donors did not impart a protective effect for recipient mice fed the TWD. Likewise, the composition of the fecal microbiomes of recipient mice was also affected to a much greater extent by the diet they consumed as opposed to the source of FMT. In summary, FMT from mice fed either basal diet with differing colitis or tumor outcomes did not shift colitis symptoms or colon tumorigenesis in recipient mice, regardless of the basal diet they consumed. These observations suggest that the gut microbiome may not contribute directly to the development of disease in this animal model.

Author ORCID Identifier

Abby D. Benninghoff https://orcid.org/0000-0002-7993-0117

Document Type

Dataset

DCMI Type

Dataset

File Format

.csv

Publication Date

1-30-2023

Funder

USDA, National Institute of Food and Agriculture (NIFA)

Utah Agricultural Experiment Station

Publisher

Utah State University

Award Number

USDA, National Institute of Food and Agriculture (NIFA) 2018-67017-27516; Utah Agricultural Experiment Station UTA-01178 and UTA-04156

Award Title

Dietary Intervention with Black Raspberries to Promote Gut Homeostasis and Prevent Colitis-Associated Colorectal Cancer

Methodology

The fecal microbiome was assessed by 16s rRNA sequencing prior to induction of colitis and tumorigenesis (pre-DSS), during active colitis (colitis), 14 days later during recovery from gut injury (recovery) at at the study end (terminal). Microbiota sequences were processed using QIIME 2 and DADA2. The DADA2 R package implements the full amplicon workflow (filtering, dereplication, chimera identification, merging paired end reads) and generates an amplicon sequence variant (ASV) table and representative sequences. To assign taxonomy, the QIIME feature-classifier classify-sklearn command was used with a classifier pre-trained for the V4 region, silva-138-99-515-806-nb-classifier.qza, and the most recent release of the Silva database (138 SSU). The resulting sequence count data are provided as ASV counts in .csv format, with the corresponding taxonomy and mapping files also in .csv format.

Language

eng

Code Lists

See README

Disciplines

Animal Sciences | Veterinary Medicine

License

This work is licensed under a Creative Commons Attribution 4.0 License.

Identifier

https://doi.org/10.26078/z54v-8j64

Recommended Citation

Benninghoff, A. (2023). Dynamic Fecal Microbiota Transfer from Mice with Western Diet-Induced Severe Colitis and Colon Tumorigenesis Did Not Exacerbate Colitis Symptoms or Colon Tumorigenesis in Recipient Mice Fed Either the AIN93G Diet or the Total Western Diet [Data set]. Utah State University. https://doi.org/10.26078/Z54V-8J64

Checksum

ee2d08b7ad0eb44f3d4f8aba21cc40ce