Aspen Bibliography

Variation in mating competence and random amplified polymorphic DNA in Laccaria bicolor (Agaricales) associated with three tree host species

Document Type

Article

Journal/Book Title/Conference

Canadian Journal of Botany

Volume

73

Issue

6

First Page

884

Last Page

890

Publication Date

1995

Abstract

We examined the population genetic structure of the ectomycorrhizal fungus Laccaria bicolor (Maire) Orton using single spore homokaryotic cultures from 33 basidiomes collected in northern Minnesota in association with red pine (Pinus resinosa Ait.), jack pine (Pinus banksiana Lamb.), and trembling aspen (Populus tremuloides Michx.) of three age-classes (0–20 years, 21–40 years, and > 41 years). Mating competence between cultures of isolates, as determined by the presence of clamp connections, revealed the presence of two subpopulations that were not freely interbreeding, one composed of 29 dikaryons, the other of 3 dikaryons. Phenetic cluster analysis using random amplified polymorphic DNA markers did not reveal differentiation between these subpopulations. Clustering failed to reveal genetically distinct groups based on incompatibility group, tree host species, or geographic origin of isolates. Key words: heterogenic incompatibility, RAPD, population genetics.

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