Aspen Bibliography
The role of ester groups in resistance of plant cell wall polysaccharides to enzymatic hydrolysis
Document Type
Article
Journal/Book Title/Conference
Appl. Biochem. Biotechnol. Clifton, N.J. : Humana Press
Volume
20
Issue
20/21
First Page
45
Last Page
61
Publication Date
1-1989
Abstract
Xylan backbones in native plant cell walls are extensively acety-lated. Previously, no direct investigations as to their role in cellulolytic enzyme resistance have been done, though indirect results point to their importance. An in vitro deesterification of aspen wood and wheat straw has been completed using hydroxylamine solutions. Yields of 90% acetyl ester removal for both materials have been accomplished, with little disruption of other fractions (i.e., lignin). Apparently, as the xylan becomes increasingly deacetylated, it becomes 5–7 times more digestible. This renders the cellulose fraction more accessible, and 2–3 times more digestible. This effect levels off near an acetyl removal of 75%, where other resistances become limiting.
Recommended Citation
Grohmann, K., Mitchell, D.J., Himmel, M.E. et al. The role of ester groups in resistance of plant cell wall polysaccharides to enzymatic hydrolysis. Appl Biochem Biotechnol 20, 45–61 (1989). https://doi.org/10.1007/BF02936472