Using Raman Spectroscopy to Detect Malignant Changes In Vivo.

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Princeton Instruments’ Technical Note

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Accurate, rapid and non-invasive detection and diagnosis of malignant disease in tissues is an important goal of biomedical research. Optical methods, such as diffuse reflectance, fluorescence spectroscopy, and Raman spectroscopy, have all been investigated as ways to attain this goal. Diffuse reflectance utilizes the absorption and scattering properties of tissues, particularly from cell nuclei and stroma. Changes in the scattering properties of tissues arise as the tissue becomes more dysplastic1, 2 due to variations in hemoglobin content3 and neovascularization.4 Fluorescence spectroscopy is also influenced by the changes in the optical properties of tissues and has been used to diagnose dysplasia.5,6.7,8 However, there are a number of disadvantages to these techniques, including the need for extensive sample preparation or excision, as well as low sensitivity and specificity rates.6,9

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