Document Type

Poster

Journal/Book Title/Conference

College of Engineering Senior Design Projects

Publisher

Utah State University

Location

Logan, UT

Publication Date

2017

Abstract

Flavonoids, which are naturally occurring in plants, are known for their many health-promoting properties1. These compounds have many applications for treatment of various health complications, but current processing limits their availability2. Research in synthetic biology and metabolic engineering has been combined to make flavonoids easier to obtain by growing them in microorganisms3. Naringenin has been of particular interest in production because it is a precursor to many other flavonoid compounds4. While much research has been done on ways to optimize the production of naringenin from the amino acid L-tyrosine, a pathway has yet to be published that takes all of that research into account. This design employs four enzymes: tyrosine ammonia lyase (TAL), 4-coumarate:CoA ligase (4CL), chalcone synthase (CHS) and chalcone isomerase (CHI) in the biosynthesis of naringenin (Figure 1). This work optimized the fermentation techniques used to produce this product biosynthetically. Fermentation conditions including carbon source, macronutrient concentrations, micronutrient concentrations, and fermentation time were evaluated.

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