Document Type
Poster
Journal/Book Title/Conference
College of Engineering Senior Design Projects
Publisher
Utah State University
Location
Logan, UT
Publication Date
2017
Abstract
Resveratrol is a phenylpropanoid with many promising health benefits as an antioxidant (1). It is produced naturally in various plant species such as grapes, peanuts, and some berries.
The production of resveratrol through plants can be a long and expensive process (2). Producing resveratrol via microorganisms could be a way to eliminate these problems and thereby lower the cost (3,4). E. coli can be used to produce this compound in several hours, compared to several months of growing plants.
This project includes the analysis of several major growth parameters in E. coli that were designed and tested to achieve the best optimization. These parameters included: media composition, temperature, induction methods, culture methods, and aeration.
These parameters were initially designed and tested using shaker flasks. After these initial tests were completed; the process was scaled up to a benchtop bioreactor to better test pH, dissolved oxygen requirement, and product production. From the data acquired during testing; scaled-up industrial size bioreactors for this process will be easy to design.
Recommended Citation
Draper, Scott; Erickson, Daniel; and Hoffmann, Brian, "Optimized Bioproduction of Resveratrol Using Genetically Modified Escherichia coli" (2017). Biological Engineering. Paper 7.
https://digitalcommons.usu.edu/bioengr_srdesign/7