Storing dry Beauveria bassiana mycelium
Document Type
Article
Journal/Book Title/Conference
International Rice Research Inst. Newsletter
Volume
13
Issue
6
Publication Date
12-1-1988
First Page
37
Last Page
38
Abstract
Several different harvesting procedures were used to obtain dry mycelium preparations of the entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana. The effects of these procedures on the survival of the fungal preparations and on their conidiation after short periods of storage at room temperature and at 4°C were examined. Harvesting procedures consisted of filtering the mycelium produced in airlift containers from the culture medium, washing with deionized water, spraying with a sugar solution, and incubating for 18 hr at 4°C before drying. Conidial production of treated mycelia stored 1.5 and 4.5 months at 4°C was not significantly different for and procedure. For dry mycelium of M. anisopliae stored 1.5 months at 4°C and then at room temperature for 3 months, maltose- and sucrose-treated preparations produced more conidia than preparations sprayed with dextrose solution, with water only, or not sprayed. B. bassiana preparations dried soon after mat formation were superior to those incubated at 4°C, and maltose-and dextrose-treated mycelia were superior to other treatments when stored at room temperature.
Recommended Citation
Rombach, M.C., R.M. Aguda and D.W. Roberts. 1988. Storing dry Beauveria bassiana mycelium. Int. Rice Res. Inst. Newsletter 13(6): 37-38