Production of Beauveria Bassiana (Deuteromycotina, Hyphomycetes), in Different Liquid Media and Subsequent Conidiation of Dry Mycelium
Mycelium ofBeauveria bassiana can be grown in liquid culture, filtered, and the mycelium dried. After rehydration the mycelium sporulates. Two carbohydrate sources (sucrose and maltose), and one nitrogen/vitamin source (yeast extract) were tested for mycelium growth and subsequent conidial production. Maximum mycelium growth (12.31 mg/ml), in liquid culture, was in the sucrose (3.5%)/yeast extract (3.5%) medium, but mycelium from a maltose (2%)/yeast extract (0.75%) medium produced the maximum of 4.62×106 conidia/mg dry mycelium after incubation in moist Petri dishes. Using the data on mycelium yield (in liquid culture) and conidial production (by dry mycelium) it is calculated that the sucrose (3.5%)/yeast extract (3.5%) and the maltose (2%)/yeast extract (0.75%) media produce most conidia per media volume (an equivalent of 3.52–3.72×107 conidia/ml).
Rombach, M.C., R.M. Aguda and D.W. Roberts. 1988. Production of Beauveria bassiana (Deuteromycotina, Hyphomycetes), in different liquid media and subsequent conidiation of dry mycelium. Entomophaga 33: 315-324.