A Procedure for Preservation of Mycelium of the Mosquito Pathogen Culicinomyces Clavisporus

Document Type

Article

Journal/Book Title/Conference

Applied Microbiology and Biotechnology

Volume

26

Issue

2

Publication Date

5-1-1987

First Page

186

Last Page

188

Abstract

An effective storage procedure has been demonstrated for the mosquito pathogen Culicinomyces clavisporus. A mycelial preparation was harvested by filtration, sprayed with a sucrose solution and air dried at 20°C in a laminar flow cabinet until the mycelial mat became crisp. This material was then ground in a hammer mill and particles of less than 355 μm were sieved out. Viability of the particles was assessed by studying mycelial growth and conidial formation when particles were added to water agar plates or to water. The particles retained 100% viability after 9 weeks storage at-20°C or 6 days at 4°C. Preparations lost activity rapidly if stored at 20°C. Conidia produced by this method were pathogenic to mosquito larvae.

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