Document Type
Poster
Publication Date
3-30-2017
Abstract
Lactococcus lactis (strain JB704) bacterial cells were transformed at 2500 Volts, 25 µF, and 200 Ω with the pTW8 vector. However, bacterial cells were grown and pre-treated differently. One culture of recipient cells was grown in MRS without 1% glycine and received no pre-treatment, were pre-treated for 30’ with water, or were pre-treated for 30’ with 100 mM lithium acetate and 10 mM dithiothreitol (DTT); a second culture of recipient cells was grown in MRS with 1% glycine and received no pre-treatment, were pretreated for 30’ with water, or were pre-treated for 30’ with 100 mM lithium acetate and 10mM dithiothreitol (DTT).
Recommended Citation
Coburn, Bryan, "Transformation of Lactic Acid Bacteria" (2017). Biology Posters. Paper 141.
https://digitalcommons.usu.edu/biology_posters/141