Date of Award:
5-2013
Document Type:
Dissertation
Degree Name:
Doctor of Philosophy (PhD)
Department:
Biology
Committee Chair(s)
Randolph V. Lewis
Committee
Randolph V. Lewis
Committee
Daryll B. DeWald
Committee
Jon Y. Takemoto
Committee
Gregory J. Podgorski
Committee
Ilka M. Nemere
Abstract
Kisspeptins are hormones involved in the onset of puberty in adolescents and maintenance of reproductive function in adults. Defects in kisspeptin signaling are associated with many reproductive disorders. Kisspeptins were originally discovered as metastasis suppressors and have been found to suppress the metastasis of certain cancers. Unfortunately, many aspects of kisspeptin action at a cellular level remain unknown. Kisspeptins, therefore, are attractive candidates for large-scale production for use as research and therapeutic reagents. We developed a simple and cost effective method to express and purify kisspeptins in large quantities. We also tested the biological activity of kisspeptins on cells expressing receptors for kisspeptins and found that those produced in our lab behave similarly to the kisspeptins synthesized by chemical means. This research was funded by Synthetic Biomanufacturing Institute, Utah State University (USU).
Checksum
f836899f1966e061bd5a8c8411569ec5
Recommended Citation
Kurian, Mathew Puthayathu, "Kisspeptins - Expression, Purification, and Biological Assays" (2013). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 1974.
https://digitalcommons.usu.edu/etd/1974
Included in
Copyright for this work is retained by the student. If you have any questions regarding the inclusion of this work in the Digital Commons, please email us at .