Date of Award:
5-2014
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Committee Chair(s)
Jon Takemoto
Committee
Jon Takemoto
Committee
Jixun Zhan
Committee
Michelle Grilley
Abstract
The BioProcessing Demonstration and Training Laboratory was established with a collaboration between Utah State University and Thermo Fisher-Scientific, Inc. This lab was developed into a fully functioning tissue culture facility. Demonstration of tissue culture procedures were necessary for the lab to be industry-fully qualified. The CHO 1-15 cell line protocols were optimized by establishing conditions for reproducibility in shaker flasks and bioreactors (2 to 250 L capacity). CHO 1-15 is the cell line of choice for protein production in the bio-manufacturing industry. Oxidative stress is a problem in the industry because it can cause a decrease in protein production. Mesobiliverdin IXα and biliverdin IXα possess antioxidant capabilities. The effects of the antioxidant nature of these compounds were tested on the CHO 1-15 cell line. Cell cultures from silkworm and spider silk producing cells were also pursued. Methods to produce a primary cell line from spider silk gland cells were developed. Cell lines from spider and silkworm silk producing glands appeared to have the capacity to secrete full-length native proteins ranging in size from 200 to 500 kDa, and possibly larger.
Checksum
310b160576808d88e9cbb94050608c04
Recommended Citation
Robinson, Susan Kathleen, "Investigations of CHO 1-15 and Silk Gland Cell Line Development" (2014). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 3690.
https://digitalcommons.usu.edu/etd/3690
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