Date of Award:
5-1992
Document Type:
Dissertation
Degree Name:
Doctor of Philosophy (PhD)
Department:
Animal, Dairy, and Veterinary Sciences
Committee Chair(s)
Thomas D. Bunch
Committee
Thomas D. Bunch
Committee
Dave Marcinkowski
Committee
Kenneth White
Committee
LeGrande Ellis
Abstract
The purpose of this research was to define the effects of various growth-promoting factors on in vitro embryonic development of in vitro matured and in vitro fertilized bovine embryos. The control medium was a chemically defined medium which improves the possibility of closely determining the in vivo conditions the embryo is actually exposed to. The growth-promoting factors tested in this experiment included transferrin, IGF-I (insulin-like growth factor-one), IGF-II (insulin-like growth factor-two), TGF-𝛼 (transforming growth factor-alpha), TGF-𝛽1 (transforming growth factor-beta1), PDGF (platelet derived growth factor), EGF (epidermal growth factor), NGF (nerve growth factor), and bFGF (basic fibroblast growth factor). Transferrin was included at 10 micrograms/milliliter, while all other factors were utilized at 10 nanograms/milliliter in the control medium.
Bovine cumulus-oocytes were retrieved from slaughterhouse ovaries and were matured in Medium-199 containing 10% fetal bovine serum for 24 hours at 39°C in a 5% CO2 atmosphere. Frozen-thawed bull sperm were swim-up separated and capacitated in medium containing heparin for 3 hours prior to insemination. Gametes were co-incubated for 18 hours and then cumulus cells were stripped from the ova. Ova which did not cleave were removed from culture 36 hours after insemination and were stained for evidence of fertilization. Embryos were cultured in one of the 10 conditions (including control) described above. A total of 150 total oocytes were cultured per treatment for a total of 10 days. EGF improved embryo development, while TGF-𝛽1 and TGF-𝛼 only slightly improved embryo development compared to the control. All other factors tested did not have a beneficial effect on embryo development in this culture medium.
In summary, EGF improved in vitro development of bovine embryos obtained from in vitro maturated and in vitro fertilized bovine oocytes. Other factors which were tested did not significantly improve in vitro bovine embryo development. Further experiments are necessary for determining the requirements of bovine embryos in vitro.
Checksum
985f600ac2a9e2c404a8a3bcadf7e004
Recommended Citation
Flood, Mark Randall, "Effect of Various Growth-Promoting Factors on Preimplantation Bovine Embryo Development in Vitro" (1992). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 4044.
https://digitalcommons.usu.edu/etd/4044
Included in
Copyright for this work is retained by the student. If you have any questions regarding the inclusion of this work in the Digital Commons, please email us at DigitalCommons@usu.edu.
