Date of Award:
5-2015
Document Type:
Dissertation
Degree Name:
Doctor of Philosophy (PhD)
Department:
Biology
Committee Chair(s)
Jon Y. Takemoto
Committee
Jon Y. Takemoto
Committee
Bradley Kropp
Committee
Dong Chen
Committee
Dennis Welker
Committee
Michelle M. Grilley
Abstract
Natural products are structurally and chemically diverse bioactive compounds produced by living organisms. The complex structures of natural products are often difficult to duplicate synthetically and thus industrial production of natural products most often depends on use of the host organisms. In this dissertation research, scalable production of natural products was pursued in bacterial platforms.
Biliverdin IXα is a natural anti-inflammatory compound which is emerging as a potent cytoprotectant in the medical field. Syringomycin E is a natural fungicide expected to be an effective agrofungicide in organic farming. Both biliverdin IXα and syringomycin E have limited supplies in the market. Genetic modification and mutation were performed to obtain bacterial strains capable of overproducing biliverdin IXα or syringomycin E. The resulting strains were grown in fermenters under optimized growth conditions for large scale production.
This research was conducted in the Department of Biology and Synthetic Bioproduct Institute at Utah State University. Novel production methods of two different natural products, biliverdin IXα and syringomycin E, were developed using bacteria as manufacturing platforms.
Checksum
a454972204df62ac8e0d98dc852248eb
Recommended Citation
Kawasaki, Yukie, "Scalable Bio-Production of High Value Products in Bacteria" (2015). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 4609.
https://digitalcommons.usu.edu/etd/4609
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