Date of Award:

5-2016

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Nutrition, Dietetics, and Food Sciences

Committee Chair(s)

Robert E. Ward

Committee

Robert E. Ward

Committee

Korry Hintze

Committee

Jerrad Legako

Abstract

Carboxymethyl-lysine (CML), an advanced glycation end product (AGEs), is formed during the cooking process and may induce toxic effects on human health. The effect of dietary CML on the plasma metabolite profile was investigated. Mice were fed with one of five different diets: AIN93 diet (negative control), a 45% kcal from fat Diet Induced Obesity diet (DIO; positive control); CML0, TWD (Total Western Diet) with low CML; CML1, TWD with medium CML, and CML2, TWD with high CML for 8 weeks, and the plasma metabolome was determined by Gas Chromatography-Mass Spectrometry (GC-MS). In addition quantitative analysis of plasma glucose level across the five diet groups was also measured using internal standard.

In the mouse plasma samples 93 compounds were detected using GC-MS. Among all 93 detected compounds, 49 of them were identified, while 44 of them could not be identified and are considered as unknowns. Four identified metabolites and 10 unknown metabolites were significantly different between the five diets. Only one metabolite, lactic acid, was significantly different between three CML diets. A principal component analysis (PCA) provided a clear separation of CML2 diet from the others. The DIO and AIN93 diets were mostly separated by PC3. Both PC1 and PC 3 affected CML0 and CML2. Glucose levels did not differ, however, CML0, CML1, and CML2 groups did showed higher glucose levels (CML0: 107.91 mg/dL, CML1: 108.46 mg/d, and CML2: 105.61 mg/dL) compared to AIN93 (104.85 mg/dL) and DIO (105.31 mg/dL).

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