Date of Award:

5-2003

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Nutrition, Dietetics, and Food Sciences

Department name when degree awarded

Nutrition and Food Sciences

Committee Chair(s)

Bart C. Weimer

Committee

Bart C. Weimer

Committee

Dr. McMahon

Committee

Dr. Anderson

Abstract

Production of volatile sulfur compounds in cheese is associated with desirable flavors. The direct source of these compounds has been assumed to arise from the metabolism of methionine and cysteine. However, the methionine concentration in cheese rises above the amount found in casein during aging, suggesting that alternative sulfur sources are present in milk. This led us to hypothesize that lactococci may acquire sulfur from the inorganic sulfur pool of milk, in addition to methionine and cysteine, to generate volatile sulfur compounds during cheese ripening.

A turbidimetric method to determine total sulfate content in milk samples was developed. The average sulfate content of milk was determined to be ~49 mg/L ± 2.0 mg/L. The limit of detection of the test was ~2.5 mg/L in Tris buffer and ~10 mg/L in milk. Skim milk samples had significantly higher total sulfate content as compared to whole milk samples.

Transport of sulfate by three strains of Lactococcus sp. was studied after we determined that milk had small, but measurable amounts of inorganic sulfate. A decrease in the environmental pH increased sulfate transport. The maximum transport occurred during exponential cellular growth phase. All strains tested had the ability to transport much more sulfate than is native in milk.

The last phase of study was to determine the metabolic fate of sulfate. Incorporation of radio-labeled sulfate into cellular protein was studied by two-dimensional gel-electrophoresis of crude cellular lysate followed by auto-radiography. Production of volatile sulfur compounds from inorganic sulfur was determined with analysis of the head space gas with gas chromatography and scintillation counting. The incorporation of radio-labeled sulfur from sulfate was not detected in proteins on two-dimensional gels. Detectable volatile sulfur compounds were found only in the case of gas chromatographic analysis of ML3 head space. However, radio-labeled volatile sulfur was detected in the head space of all the three strains with scintillation counting. This study defined that lactococci can fix inorganic sulfur into volatile sulfur compounds in small amounts.

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