Date of Award:
5-1978
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Chemistry and Biochemistry
Committee Chair(s)
Elizabeth A. Boeker
Committee
Elizabeth A. Boeker
Committee
Thomas Emery
Committee
Joseph Street
Abstract
The purpose of this paper was to develop a single assay system for the enzymes which regulate GABA and glutamate concentrations in brain and nerve tissue. Since all the enzymes produce L-glutamate, their activities were measured by coupling them to L-glutamate decarboxylase. Enzymatic activity was determined by measuring the release of co2 from radioactive substrates. The glutamate decarboxylase was obtained from a commercial acetone powder by simplifying existing procedures. The glutamate decarboxylase produced was of sufficient purity to be used in the coupled assays, which were checked with commercial preparations of each enzyme, where available, and with crude brain homogenates. All of the assays were shown to be linear with respect to both time and enzyme concentration, thus assuring the feasibility of the technique.
Checksum
4e1c5ad4132f0d4fe3bd78764282c4f4
Recommended Citation
Dinwoodie, Robert C., "A Unified Radiometric Assay System for the Gaba-Glutamate Regulating Enzymes" (1978). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 7179.
https://digitalcommons.usu.edu/etd/7179
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