Date of Award:
5-1971
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Plants, Soils, and Climate
Department name when degree awarded
Botany
Committee Chair(s)
M. Coburn Williams
Committee
M. Coburn Williams
Committee
Wade H. Dewey
Committee
W. S. Boyle
Abstract
DMSO (dimethyl sulfoxide) and propham (isopropyl carbanilate) were investigated to determine their effect singly and in combination on mitoses in rye (Secale cereale) root tip cells. Root tips were prepared for observation by the paraffin and aceto-carmine squash methods. Results showed DMSO caused the following abnormalities: arrested metaphases, multipolar spindles, multinucleate cells, stickiness, shortened chromosomes, and mitotic inhibition. Cells from propham treated root tips exhibited the same abnormalities as those treated with DMSO. In addition, propham treatments resulted in polyploid cells and chromosome granulation. In combination, DMSO and propham caused all the abnormalities mentioned above except polyploidy. In the combination abnormalities were more frequent and occurred more rapidly.
Checksum
e779530330f7c9cb1d5442007982caf2
Recommended Citation
Rosequist, Max, "Effects of DMSO and Propham on Mitosis in Root Tips of Secale cereale" (1971). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 8273.
https://digitalcommons.usu.edu/etd/8273
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