Date of Award:
5-1977
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Committee Chair(s)
James T. Bowman
Committee
James T. Bowman
Committee
John R. Simmons
Committee
Raymond Lynn
Committee
Roger Wyse
Abstract
Soybean (Glycine max L. c.v. Consoy) tissue cultures were grown in synthetic media containing various concentrations and combinations of minerals, auxins, cytokinins, pH levels, and inorganic growth factors in a systematic procedure to establish optimum media for initiation and maintenance of callus. Further modification of the hormone level was employed to initiate callus morphogenesis. Normally toxic levels of atrazine were established in soybean calli media, and ethyl methanesulfonate (EMS) and X ray-induced mutant calli were selected for atrazine tolerance.
Optimal media for initiation and maintenance of soybean calli were developed and a defined medium was determined that consistently yields root differentiation in nearly 25% of the trials. Atrazine concentrations of 8 mg/l or greater in the medium resulted in 100% fatality of soybean calli; however, approximately 2% of calli treated with X rays or EMS demonstrated resistance to atrazine at a concentration of 20 mg/l. The implications and potential uses of these developments are discussed.
Checksum
7c6f8fd06e0de51cf64468aea0026fea
Recommended Citation
WIlson, Thomas Daniel, "In vitro Morphogenesis and Mutation of Glycine max L." (1977). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 8293.
https://digitalcommons.usu.edu/etd/8293
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