Date of Award:
5-2002
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Committee Chair(s)
Daryll B. DeWald
Committee
Daryll B. DeWald
Committee
Jon Y. Takemoto
Committee
Gregory J. Podgorski
Committee
Marie K. Walsh
Abstract
In all eukaryotic organisms vesicle-mediated protein transport is crucial for maintaining cellular homeostasis. Over the past decade significant strides have been made in elucidating the molecular mechanisms governing this complex process. Both genetic and biochemical studies have demonstrated the unequivocal involvement of class III phospatidylinositol 3-kinases (PtdIns 3-kinases) in regulating vesicle transport. While PtdIns 3-kinases have been well studied in both yeast and mammalian systems, the role of these enzymes in plants is poorly understood. In the present study, we demonstrate that the Arabidopsis Ptdlns 3-kinase is localized to the cytoplasm, and perhaps the Golgi apparatus. Moreover, we show that reduction in cellular PtdIns(3)P levels is correlated with missorting of a vacuolar marker protein. Altogether, our data suggest that AtVps34p (and its lipid product PtdIns(3)P) is involved in regulating vesicle-mediated protein transport to the vacuole in Arabidopsis.
Checksum
22fa682f8d0638ec236909fc3cd7f791
Recommended Citation
Jones, Christopher A., "The Role of the Arabidopsis Phosphatidylinositol 3-Kinase in Vacuolar Protein Transport" (2002). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 8300.
https://digitalcommons.usu.edu/etd/8300
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