Date of Award:
5-1972
Document Type:
Dissertation
Degree Name:
Doctor of Philosophy (PhD)
Department:
Biology
Department name when degree awarded
Bacteriology and Public Health
Committee Chair(s)
Rex S. Spendlove
Committee
Rex S. Spendlove
Committee
Larre N. Egbert
Committee
Paul B. Carter
Committee
Jay O. Jensen
Committee
Terry Alger
Committee
John J. Skujins
Abstract
The commonly used techniques for viral detection are tedious, time consuming and in many cases inadequate. As a consequence, a rapid, sensitive radioimmunoassay has been developed for detecting viruses. Reovirus is reacted with homologous 125I labeled antibody after which the antigen-antibody complexes are separated from unreacted labeled antibody by density gradient ultracentrifugation. After centrifugation, the density gradient is fractionated and the radioactivity counted in a liquid scintillation spectrometer.
The amount of activity in the lower fractions of the density gradient is directly proportional to the virus concentration. The radioimmunoassay developed has several advantages over other viral assay procedures; the method is rapid, viral samples can be assayed within six hours after receiving the sample, both viable and inactive viruses are detected, and the procedure is sensitive.
Checksum
101302ecca3a0e5fe1babae2478cbd0d
Recommended Citation
Perez, John Carlos, "Radioimmuno Detection of Virus" (1972). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 8347.
https://digitalcommons.usu.edu/etd/8347
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