Date of Award:

5-1989

Document Type:

Thesis

Degree Name:

Master of Science (MS)

Department:

Chemistry and Biochemistry

Committee Chair(s)

Joseph K.-K. Li

Committee

Joseph K.-K. Li

Committee

T. A. Grover

Committee

B. R. Copeland

Abstract

BTV-2, 10, 11, 13 and 17 were purified by three discontinuous sucrose gradients. Viral proteins were separated from the genomic double-stranded RNA using a newly developed KCI-SDS (sodium dodecyl sulfate) precipitation method. Cyanogen bromide (CNBr) peptide mapping of VP5 and VP7 from bluetongue virus (BTV) serotypes 2, 10, 11, 13 and 17 was then done using silver-stained SDS-polyacrylamide gels. The antigenic epitopes of VP5 and VP7 digested with CNBr were also investigated with polyclonal antibodies produced against KCl-SCS precipitated BTV-13 viral proteins. The peptide maps and the antigenic epitopes of VP7 were similar among the five American serotypes with very minor differences detected in the VP7 of BTV-13. However, the peptide and epitope patterns of VP5 showed more variation among these five U.S. serotypes. These results were used to deduce the genetic relatedness of the genome segments M2 and S1 coding for VP5 and VP7, respectively, among these five American serotypes.

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