Date of Award:
5-1972
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Plants, Soils, and Climate
Department name when degree awarded
Botany
Committee Chair(s)
G. W. Cochran
Committee
G. W. Cochran
Committee
O. S. Cannon
Committee
D. L. Mumford
Committee
W. Dewey
Abstract
A small tobacco mosaic virus nucleoprotein unit, thought to be the basic infectious unit of TMV, was isolated and purified from systemically-infected Turkish tobacco leaves. This small TMV particle was characterized by a doughnut shaped form as shown in the electron micrographs and by the induction of gray lesions when inoculated on Scotia bean leaves. Four carbodiimides, organic chemical condensing reagents, were compared for effectiveness in inducing polymerization of this starting material into longer rod-shaped viral particles. Dicyclohexyl carbodiimide, a wax-like solid, was found to be the most suitable reagent when used with an organic solvent such as ethanol in order to avoid the generation of a two-phase system in the reaction tube.
After reaction with the carbodiimide the polymerized TMV particles induced almost typical brown lesions on Scotia bean leaves and the length of the particles varied from 60 to 300 nm.
Recommended Citation
de Tosso, Alicia Bruna, "An Isolation and Partial Purification of a Small Tobacco Mosaic Virus Nucleoprotein Unit and a Study of its Polymerization by Carbodiimides" (1972). All Graduate Theses and Dissertations, Spring 1920 to Summer 2023. 9105.
https://digitalcommons.usu.edu/etd/9105
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