Date of Award:

5-1-1987

Document Type:

Dissertation

Degree Name:

Doctor of Philosophy (PhD)

Department:

Biology

Department name when degree awarded

Biology

Committee Chair(s)

Anne J. Anderson

Committee

Anne J. Anderson

Committee

Joseph K.-K. Li

Committee

Neal Van Alfen

Committee

Jeffery K. Kondo

Committee

Jim Bowman

Abstract

Certain extracellular components from the α race of Colletotrichum Iindemuthlanum exhibit differential elicitor activity in bean cultivars. Purification of a race extracellular components by a variety of chromatographic techniques has revealed elicitor activity in components with different chemical compositions. One class of elicitors, which does not adsorb to DEAE-Sephadex or CM-Sephadex is a carbohydrate-rich complex containing galactose (17%), glucose (38%), and mannose (45%). This purified elicitor induced high accumulations of phenolics, phytoalexins (kievitone, phaseollinisoflavan and phaseollin) and mRNA's corresponding to the enzymes phenylalanine ammonia-lyase and chalcone synthase in incompatible bean cultivars (Dark Red Kidney and Tennessee Green Pod). Very little elicitor activity was displayed on a compatible bean cultivar (Great Northern). No extracellular components from the β race exhibited elicitor activity on β race-compatible Dark Red Kidney and Great Northern bean.

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