Date of Award:
5-1-1990
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Department name when degree awarded
Biology
Committee Chair(s)
Bill B. Barnett
Committee
Bill B. Barnett
Committee
Robert W. Sidwell
Committee
Ronald C. Sims
Abstract
Viruses may be concentrated from culinary water by adsorption to filters. The quantification of these adsorbed viruses requires that they first be eluted from the filters. The elution of poliovirus and reovirus from charge-modified filters was studied in this work. This report describes the effect of varying the pH and composition of several different elution buffers upon the efficiency of virus elution from Virozorb 1 MDS filters. Presumably, the attraction between the virus and the filter media is through ionic interaction or hydrogen bonding or both. To neutralize ionic attraction forces, elution buffer pH was varied so that both the virus and filter carried the same ionic charge. The nonionic detergent, monolaurate polyoxyethylenesorbitan (Tween 20), was used to disrupt hydrogen bonding. These studies demonstrated that ionic charge plays the major role in virus elution, while hydrogen bonding plays a lesser role. The elution buffer that produced optimum recovery for both poliovirus and reovirus was a solution of 3% beef extract with 0.05% Tween 20 at pH 9.0, which resulted in elution efficiencies of 95% for poliovirus and 94% for reovirus.
Recommended Citation
Park, Kum-Jae, "The Development and Evaluation of Procedures for Recovering Polioviruses and Reoviruses From Culinary Waters Using Electropositively Charged Filters" (1990). Biology. 577.
https://digitalcommons.usu.edu/etd_biology/577
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