Date of Award:
5-1-1996
Document Type:
Thesis
Degree Name:
Master of Science (MS)
Department:
Biology
Committee Chair(s)
Anne J. Anderson
Committee
Anne J. Anderson
Committee
Joan E. McLean
Committee
David B. Drown
Committee
Ronald C. Sims
Abstract
Successful bioremediation in soils using Phanerochaete chrysosporium is determined by many soil factors. Several biotic and abiotic soil factors inhibited P. chrysosporium in the mineralization of pyrene when compared to a non-soil system. Inhibition of pyrene mineralization was also observed with layers of sterilized silt and clay but not sand. Two clays, bentonite and kaolinite, differentially sorbed and decreased the mineralization of pyrene. Four artificial sterile, nonorganic soils, made with different proportions of sand, silt, and clay, inhibited pyrene mineralization by P. chrysosporium. Both purified humic acid and manganese oxide significantly reduced the mineralization potential of P. chrysosporium. MnO2 (Mn+4) was found to eliminate the growth of P. chrysosporium at a concentration of 250 mg/kg when amended to potato dextrose agar plates, whereas MnCl2 (Mn+2) was inhibitory at concentrations greater than 1000 mg/Kg. Soils treated with hydrogen peroxide reduced the efficacy of P. chrysosporium to mineralize pyrene, and enhanced sporulation of the fungus. Several bacteria isolated from an inhibitory Timpanogos series soil were antagonistic to the growth of P. chrysosporium on solid medium. Bacteria inhibited mineralization of pyrene by the fungus when co-inoculated into sterile soil. A native soil in the Kidman series, without inoculation, degraded pyrene to a greater extent than when it was inoculated with P. chrysosporium. In certain soils, bioremediation by P. chrysosporium could be suppressed by the abiotic features of the soil composition as well as by indigenous soil microorganisms. Also, the addition of the fungus/substrate may reduce overall bioremediation of soil contaminates by native microorganisms.
Recommended Citation
Tucker, Brian J., "Influence of Soil on Bioremediation Potential of Phanerochaete chrysosporium" (1996). Biology. 630.
https://digitalcommons.usu.edu/etd_biology/630
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